Hotspot

rps16-trnQ-UUG

LSC · 6259-6958 · 700 bp

Back to Bauhinia

Pi 0.0464

MarkerSeek score 74.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 100
pi: 0.0464
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGATGTCGAGTCAAGAGC	CGAAAACTTACAGCGGCTTG	50.0	50.0	58.0	58.0	1468-1583 bp	1.0	91.5
rps16-trnQ-UUG_p2	AACGATGTCGAGTCAAGAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	802-877 bp	1.0	91.3
rps16-trnQ-UUG_p3	AACGATGTCGAGTCAAGAGC	ATCGAAAACTTACAGCGGCT	50.0	45.0	58.0	57.9	1470-1585 bp	1.0	91.1
rps16-trnQ-UUG_p4	AACGATGTCGAGTCAAGAGC	TCGAAAACTTACAGCGGCTT	50.0	45.0	58.0	58.1	1469-1584 bp	1.0	90.9
rps16-trnQ-UUG_p5	AAGTTTTCTCCTCGTACGGC	CGAAAACTTACAGCGGCTTG	50.0	50.0	57.9	58.0	2080-2193 bp	1.0	90.7