Hotspot

trnH-GUG-psbA

LSC · 87-456 · 370 bp

Back to Bauhinia

Pi 0.0539

MarkerSeek score 72.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 61
pi: 0.0539
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	427-439 bp	1.0	90.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CGGTCGAGGCTCCATCTATA	55.0	55.0	58.1	58.2	408-420 bp	1.0	89.5
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	476-488 bp	1.0	88.3
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	477-489 bp	1.0	87.8
trnH-GUG-psbA_p5	GCCTTGATCCACTTGGCTAC	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.6	57.9	432-444 bp	1.0	86.5