Hotspot

ycf1

SSC · 122329-127899 · 5571 bp

Back to Bassia

Pi 0.0297
MarkerSeek score 64.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5571
variable_sites
359
pi
0.0297
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GCTCTACCCACATGTCGTTT CAGCAAGAAGAGGGATGCAT 50.0 50.0 57.9 57.9 1171-1183 bp 0.6 63.4
ycf1_p2 GATGCTCTACCCACATGTCG CAGCAAGAAGAGGGATGCAT 55.0 50.0 58.1 57.9 1174-1186 bp 0.6 63.0
ycf1_p3 GCTCTACCCACATGTCGTTT AACAGCAAGAAGAGGGATGC 50.0 50.0 57.9 58.2 1173-1185 bp 0.6 62.4
ycf1_p4 TCAAAACGATATCGCCACGT CAGCAAGAAGAGGGATGCAT 45.0 50.0 58.0 57.9 1288 bp 0.2 47.6
ycf1_p5 TCAAAACGATATCGCCACGT AACAGCAAGAAGAGGGATGC 45.0 50.0 58.0 58.2 1290 bp 0.2 46.6