Hotspot

rps3

LSC · 82523-83182 · 660 bp

Back to Bassia

Pi 0.013
MarkerSeek score 45.90
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
660
variable_sites
18
pi
0.013
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 AGTCGTTATTGGTCCGAAGC CGAGCTCGAGGACGTAGTTA 50.0 55.0 58.0 58.7 1490-1576 bp 1.0 77.9
rps3_p2 GTCGTTATTGGTCCGAAGCT CGAGCTCGAGGACGTAGTTA 50.0 55.0 58.0 58.7 1489-1575 bp 1.0 77.9
rps3_p3 TGTGATGCGATGAGTTGGTT CGAGCTCGAGGACGTAGTTA 45.0 55.0 57.8 58.7 1180-1265 bp 1.0 76.7
rps3_p4 AGTCGTTATTGGTCCGAAGC TCTCACTCCGGAACATTTCC 50.0 50.0 58.0 57.2 1386-1387 bp 0.6 58.2
rps3_p5 GTCGTTATTGGTCCGAAGCT TCTCACTCCGGAACATTTCC 50.0 50.0 58.0 57.2 1385-1386 bp 0.6 58.2