Hotspot

ndhF-rpl32

SSC · 110837-111882 · 1046 bp

Back to Bassia

Pi 0.03
MarkerSeek score 68.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1046
variable_sites
64
pi
0.03
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGGTATGATCCATGCATATTGA ACGCTTTTTGTAAATTGAAGTACG 39.1 33.3 57.2 57.0 1125-1136 bp 1.0 45.0
ndhF-rpl32_p2 AGGGTATGATCCATGCATATTGA CGCTTTTTGTAAATTGAAGTACGT 39.1 33.3 57.2 57.0 1124-1135 bp 1.0 45.0
ndhF-rpl32_p3 AAGGGTATGATCCATGCATATTGA ACGCTTTTTGTAAATTGAAGTACG 37.5 33.3 57.9 57.0 1126-1137 bp 1.0 45.0
ndhF-rpl32_p4 AAGGGTATGATCCATGCATATTGA CGCTTTTTGTAAATTGAAGTACGT 37.5 33.3 57.9 57.0 1125-1136 bp 1.0 45.0
ndhF-rpl32_p5 AGGGTATGATCCATGCATATTGA CGCTTTTTGTAAATTGAAGTACGTT 39.1 32.0 57.2 57.6 1124-1135 bp 1.0 45.0