Hotspot

trnN-GUU-ndhF

IRb · 107079-108610 · 1532 bp

Back to Bassia

Pi 0.0722
MarkerSeek score 85.20
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1532
variable_sites
259
pi
0.0722
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 CGGGAGAATCAATGACTGCA TGGAGGAGGAGGTCGAATTT 50.0 50.0 58.0 58.0 1964-2317 bp 1.0 90.6
trnN-GUU-ndhF_p2 GGCAGAATAGCAGAGCAAGT TGGAGGAGGAGGTCGAATTT 50.0 50.0 58.0 58.0 2060-2413 bp 1.0 90.4
trnN-GUU-ndhF_p3 CGGGAGAATCAATGACTGCA GTATGGAGGAGGAGGTCGAA 50.0 55.0 58.0 57.9 1967-2320 bp 1.0 90.4
trnN-GUU-ndhF_p4 CGGGAGAATCAATGACTGCA AAGTATGGAGGAGGAGGTCG 50.0 55.0 58.0 57.9 1969-2322 bp 1.0 90.3
trnN-GUU-ndhF_p5 TGGGTCTTTTGTCGAAGTGG TGGAGGAGGAGGTCGAATTT 50.0 50.0 58.0 58.0 2207-2560 bp 1.0 90.2