Hotspot

rpl22-rps19

LSC · 83830-83894 · 65 bp

Back to Bassia

Pi 0.0477
MarkerSeek score 65.40
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
65
variable_sites
6
pi
0.0477
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCGTGCTTTGTCAACAGACA AAGCTTAACACAAAGGCGGA 45.0 45.0 58.0 58.0 469-473 bp 1.0 84.6
rpl22-rps19_p2 TCGTGCTTTGTCAACAGACA AGCTTAACACAAAGGCGGAA 45.0 45.0 58.0 58.0 468-472 bp 1.0 84.6
rpl22-rps19_p3 CAAGTCGGAAACCAAGTGGA TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.0 58.0 1318-1401 bp 1.0 83.8
rpl22-rps19_p4 TCGTGCTTTGTCAACAGACA TGGGTAGAGCCGGATCTAAG 45.0 55.0 58.0 58.0 819-825 bp 1.0 83.4
rpl22-rps19_p5 TCGTGCTTTGTCAACAGACA CGTCCCGTAGTAAGAGGAGT 45.0 55.0 58.0 58.0 784-790 bp 1.0 83.3