Hotspot

trnS-GGA-rps4

LSC · 45065-45413 · 349 bp

Back to Bassia

Pi 0.0327
MarkerSeek score 65.00
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
349
variable_sites
24
pi
0.0327
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 AGGCTTTTGTTTACCGAGGG AGTAGAATATTATTCTCGTCAGACT 50.0 32.0 58.1 54.9 416-437 bp 1.0 44.9
trnS-GGA-rps4_p2 AGGCTTTTGTTTACCGAGGG AGAATATTATTCTCGTCAGACTT 50.0 30.4 58.1 52.8 413-434 bp 1.0 44.9
trnS-GGA-rps4_p3 GGCTTTTGTTTACCGAGGGT AGTAGAATATTATTCTCGTCAGACT 50.0 32.0 58.4 54.9 415-436 bp 1.0 44.9
trnS-GGA-rps4_p4 AGGCTTTTGTTTACCGAGGG AGTAGAATATTATTCTCGTCAGACTT 50.0 30.8 58.1 55.6 416-437 bp 1.0 44.9
trnS-GGA-rps4_p5 AGGCTTTTGTTTACCGAGGG GTAGAATATTATTCTCGTCAGACT 50.0 33.3 58.1 53.6 415-436 bp 1.0 44.9