Hotspot

petN-psbM

LSC · 28238-28961 · 724 bp

Back to Bassia

Pi 0.0186
MarkerSeek score 64.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
724
variable_sites
28
pi
0.0186
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATTGTAAGTCTCGCTTGGGC AAAACAGTCAGCCAAAATGA 50.0 35.0 58.3 53.9 829-841 bp 1.0 49.3
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT AAAACAGTCAGCCAAAATGA 50.0 35.0 57.6 53.9 767-779 bp 1.0 48.9
petN-psbM_p3 ATTGTAAGTCTCGCTTGGGC TGTAAAAACAGTCAGCCAAAATGA 50.0 33.3 58.3 57.8 833-845 bp 1.0 48.5
petN-psbM_p4 ATTGTAAGTCTCGCTTGGGC AAAAACAGTCAGCCAAAATGA 50.0 33.3 58.3 54.8 830-842 bp 1.0 48.3
petN-psbM_p5 ATTGTAAGTCTCGCTTGGGC TGCTACTGCACTATTCATTCT 50.0 38.1 58.3 54.7 884-896 bp 1.0 47.9