Hotspot

atpA-atpF

LSC · 11485-11547 · 63 bp

Back to Bassia

Pi 0.054
MarkerSeek score 65.30
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
63
variable_sites
8
pi
0.054
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 GGGCGCAGGAGATTCAATTA GTAATTGAGCGTGAGAGCCA 50.0 50.0 58.0 58.0 976-978 bp 1.0 82.1
atpA-atpF_p2 GGGCGCAGGAGATTCAATTA TCAAGTTTGGTTCGGGAAGG 50.0 50.0 58.0 58.0 941-943 bp 1.0 82.0
atpA-atpF_p3 GGGCGCAGGAGATTCAATTA TCAGAAGAACTTCGCGGAAG 50.0 50.0 58.0 57.9 823-825 bp 1.0 81.8
atpA-atpF_p4 CACCGGCCATTACTTCATCA GTAATTGAGCGTGAGAGCCA 50.0 50.0 58.0 58.0 727 bp 0.8 73.8
atpA-atpF_p5 CACCGGCCATTACTTCATCA TCAAGTTTGGTTCGGGAAGG 50.0 50.0 58.0 58.0 692 bp 0.8 73.8