Hotspot

trnK-UUU-rps16

LSC · 4223-4959 · 737 bp

Back to Bassia

Pi 0.0258
MarkerSeek score 61.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
737
variable_sites
38
pi
0.0258
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCGGGTTTCTTGGGTTATC GCCGTACGAGGAGAAAACTT 50.0 50.0 57.9 57.9 1709-1751 bp 1.0 83.9
trnK-UUU-rps16_p2 CCTCGGAGGCAGGTAAATTC GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1648-1673 bp 0.8 74.7
trnK-UUU-rps16_p3 CCTCGGAGGCAGGTAAATTC TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 1593-1618 bp 0.8 74.6
trnK-UUU-rps16_p4 CCTCGGAGGCAGGTAAATTC AGCCGTCTATCGAATCGTTG 55.0 50.0 58.0 57.9 1581-1606 bp 0.8 74.3
trnK-UUU-rps16_p5 CCTCGGAGGCAGGTAAATTC GGGGGTCTTGCTGATTCAAT 55.0 50.0 58.0 57.9 1612-1615 bp 0.6 63.5