Hotspot

trnH-GUG-psbA

LSC · 80-369 · 290 bp

Back to Bassia

Pi 0.0389
MarkerSeek score 71.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
290
variable_sites
22
pi
0.0389
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTGGACCTAGCTGCTAT 55.0 55.0 58.1 58.0 340-357 bp 1.0 88.2
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTGGACCTAGCTGCTAT 50.0 55.0 57.9 58.0 354-371 bp 1.0 87.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTGGACCTAGCTGCTAT 50.0 55.0 57.9 58.0 353-370 bp 1.0 87.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTGGACCTAGCTGCTAT 50.0 55.0 57.9 58.0 355-372 bp 1.0 87.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTTCAGGCTGAGCACAACAT 55.0 45.0 58.1 58.0 802-819 bp 1.0 83.5