Hotspot

trnD-GUC

LSC · 32384-32457 · 74 bp

Back to Barringtonia

Pi 0.027

MarkerSeek score 68.00

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 2
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	ACAGAAGGGAGAGGGTTGAA	TAACTCTTTGTCCTGCAGGA	50.0	45.0	57.9	56.1	195 bp	1.0	64.6
trnD-GUC_p2	TTCACAGAAGGGAGAGGGTT	TAACTCTTTGTCCTGCAGGA	50.0	45.0	57.9	56.1	198 bp	1.0	64.6
trnD-GUC_p3	TTTCACAGAAGGGAGAGGGT	TAACTCTTTGTCCTGCAGGA	50.0	45.0	57.9	56.1	199 bp	1.0	64.6
trnD-GUC_p4	ACAGAAGGGAGAGGGTTGAA	CCTGCAGGAATACCTAATACGG	50.0	50.0	57.9	58.1	184 bp	1.0	63.3
trnD-GUC_p5	TTCACAGAAGGGAGAGGGTT	CCTGCAGGAATACCTAATACGG	50.0	50.0	57.9	58.1	187 bp	1.0	63.3