Hotspot

ndhJ

LSC · 51736-52212 · 477 bp

Back to Barringtonia

Pi 0
MarkerSeek score 30.50
Primer available yes
Label ndhJ

Feature Metadata

label_name
ndhJ
feature_type
gene
region
LSC
length_bp
477
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ_p1 AATCGCGAACAGAGTGTACA ACAAATCAAAATGCGGGAGAG 45.0 42.9 57.3 57.1 592 bp 1.0 59.4
ndhJ_p2 ATCGCGAACAGAGTGTACAA ACAAATCAAAATGCGGGAGAG 45.0 42.9 57.3 57.1 591 bp 1.0 59.4
ndhJ_p3 TCGCGAACAGAGTGTACAAT ACAAATCAAAATGCGGGAGAG 45.0 42.9 57.3 57.1 590 bp 1.0 59.4
ndhJ_p4 ATCGCGAACAGAGTGTACAA ACAAATCAAAATGCGGGAGA 45.0 40.0 57.3 55.9 591 bp 1.0 57.6
ndhJ_p5 TCGCGAACAGAGTGTACAAT ACAAATCAAAATGCGGGAGA 45.0 40.0 57.3 55.9 590 bp 1.0 57.6