Hotspot

ndhJ-ndhK

LSC · 52213-52328 · 116 bp

Back to Barringtonia

Pi 0.00862

MarkerSeek score 57.50

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 1
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CTAGCCAAGCAGACAAACGA	ACCGCCATCTACTTCAGAGA	50.0	50.0	58.2	57.9	226 bp	1.0	77.6
ndhJ-ndhK_p2	GCCAATCCTCGGGCTTTATT	ACCGCCATCTACTTCAGAGA	50.0	50.0	58.3	57.9	307 bp	1.0	76.6
ndhJ-ndhK_p3	CTTGACTAGCCAAGCAGACA	ACCGCCATCTACTTCAGAGA	50.0	50.0	57.5	57.9	231 bp	1.0	75.5
ndhJ-ndhK_p4	ACTAGCCAAGCAGACAAACG	ACCGCCATCTACTTCAGAGA	50.0	50.0	58.5	57.9	227 bp	1.0	75.3
ndhJ-ndhK_p5	TAGCCAAGCAGACAAACGAC	ACCGCCATCTACTTCAGAGA	50.0	50.0	58.5	57.9	225 bp	1.0	75.3