Hotspot

psbM-trnD-GUC

LSC · 31249-32383 · 1135 bp

Back to Barringtonia

Pi 0

MarkerSeek score 26.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGACGCGAAATTGGAATCCT	CATAGTATGATGGCGGTCGG	45.0	55.0	57.6	58.0	1752 bp	1.0	76.8
psbM-trnD-GUC_p2	AGACGCGAAATTGGAATCCT	AGTGATTCTTCCTGGGTCGA	45.0	50.0	57.6	58.1	1605 bp	1.0	76.4
psbM-trnD-GUC_p3	AGACGCGAAATTGGAATCCT	AAGTATGCCCCCATCGTCTA	45.0	50.0	57.6	57.9	1730 bp	1.0	76.1
psbM-trnD-GUC_p4	AGACGCGAAATTGGAATCCT	TCTCTTTCAAGGAGGCAACG	45.0	50.0	57.6	57.8	1695 bp	1.0	75.5
psbM-trnD-GUC_p5	AGACGCGAAATTGGAATCCT	TGGCAATATGTCTACGCTGG	45.0	50.0	57.6	57.8	1548 bp	1.0	75.0