Hotspot

trnS-GCU-trnG-GCC

LSC · 9079-9776 · 698 bp

Back to Barringtonia

Pi 0.00573
MarkerSeek score 62.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
698
variable_sites
4
pi
0.00573
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 804 bp 1.0 67.3
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 803 bp 1.0 67.3
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCCTTAACT 50.0 45.0 58.2 56.4 858 bp 1.0 66.1
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 805 bp 1.0 65.4
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 804 bp 1.0 65.4