Hotspot

rps16-trnQ-UUG

LSC · 6152-7699 · 1548 bp

Back to Barringtonia

Pi 0
MarkerSeek score 58.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1548
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTTCTTCAAGTCGCACGTTG GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1685-1686 bp 1.0 79.9
rps16-trnQ-UUG_p2 ACCACATCGTTTCAACCGAA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.0 58.0 1659-1660 bp 1.0 79.6
rps16-trnQ-UUG_p3 GTTCTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1627-1628 bp 1.0 79.4
rps16-trnQ-UUG_p4 ACCACATCGTTTCAACCGAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 1601-1602 bp 1.0 79.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1678-1679 bp 1.0 78.9