Hotspot

ycf1

SSC · 122710-127998 · 5289 bp

Back to Barnadesia

Pi 0.00305

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5289
variable_sites: 16
pi: 0.00305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTAGAGCAATTTTGGACC	CGCCAGGAATCAAAACAAGTC	40.9	47.6	56.9	58.1	199 bp	1.0	47.7
ycf1_p2	GTTGTAGAGCAATTTTGGACCT	CGCCAGGAATCAAAACAAGTC	40.9	47.6	56.9	58.1	198 bp	1.0	47.7
ycf1_p3	AGTTGTAGAGCAATTTTGGACC	CGCCAGGAATCAAAACAAGT	40.9	45.0	56.9	56.9	199 bp	1.0	47.6
ycf1_p4	GTTGTAGAGCAATTTTGGACCT	ACGCCAGGAATCAAAACAAG	40.9	45.0	56.9	56.9	199 bp	1.0	47.6
ycf1_p5	GTTGTAGAGCAATTTTGGACCT	CGCCAGGAATCAAAACAAGT	40.9	45.0	56.9	56.9	198 bp	1.0	47.6