Hotspot

rps8

LSC · 79525-79929 · 405 bp

Back to Barnadesia

Pi 0.00494

MarkerSeek score 47.30

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 2
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TATTAGACAACCCGCCCCTT	CAAATTTGGAGGCACTAATAA	50.0	33.3	58.4	52.1	513 bp	1.0	40.3
rps8_p2	CCCGCCCCTTTTCTTTTCTT	CAAATTTGGAGGCACTAATAA	50.0	33.3	58.7	52.1	503 bp	1.0	40.3
rps8_p3	AACCCTATTAGACAACCCGC	CAAATTTGGAGGCACTAATAA	50.0	33.3	57.3	52.1	518 bp	1.0	40.3
rps8_p4	TATTAGACAACCCGCCCCTT	CAAATTTGGAGGCACTAATAAT	50.0	31.8	58.4	52.8	513 bp	1.0	40.3
rps8_p5	CTATTAGACAACCCGCCCCT	CAAATTTGGAGGCACTAATAA	55.0	33.3	58.9	52.1	514 bp	1.0	40.3