Hotspot

ndhF-rpl32

SSC · 110985-112067 · 1083 bp

Back to Barnadesia

Pi 0.0105

MarkerSeek score 67.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1083
variable_sites: 11
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	GCTTTTAACGCCGCCAAATA	50.0	45.0	58.0	57.7	1243-1273 bp	1.0	80.9
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	GCTTTTAACGCCGCCAAATA	45.0	45.0	58.0	57.7	1241-1271 bp	1.0	80.8
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	GCTTTTAACGCCGCCAAATA	45.0	45.0	58.0	57.7	1242-1272 bp	1.0	80.8
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	GCCGCCAAATATCCTTTCCT	50.0	50.0	58.0	58.3	1234-1264 bp	1.0	80.5
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	GCCGCCAAATATCCTTTCCT	45.0	50.0	58.0	58.3	1232-1262 bp	1.0	80.5