Hotspot

accD-psaI

LSC · 58147-58834 · 688 bp

Back to Barnadesia

Pi 0.00449

MarkerSeek score 64.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 3
pi: 0.00449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	849-869 bp	1.0	68.1
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	848-868 bp	1.0	68.1
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	850-870 bp	1.0	68.1
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.0	58.0	849-869 bp	1.0	66.2
accD-psaI_p5	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	839-859 bp	1.0	66.1