Hotspot

ycf1

SSC · 122050-127449 · 5400 bp

Back to Barleria

Pi 0.0245

MarkerSeek score 58.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5400
variable_sites: 253
pi: 0.0245
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTTTCCATCACTTTGGCA	GCAGAATACCGTCACCCATT	45.0	50.0	58.0	58.0	370-376 bp	0.8	79.3
ycf1_p2	TCGTTTCCATCACTTTGGCA	ATTATGGACCCGCTACCAGA	45.0	50.0	58.0	57.9	739-745 bp	0.8	75.4
ycf1_p3	TCGTTTCCATCACTTTGGCA	GGCCCTCCTATCTCTTCCTT	45.0	55.0	58.0	57.9	961-967 bp	0.8	74.3
ycf1_p4	TTCATGGGTCCTGGTTCTCT	GCAGAATACCGTCACCCATT	50.0	50.0	58.0	58.0	883-898 bp	0.6	67.2
ycf1_p5	TTCATGGGTCCTGGTTCTCT	ATTATGGACCCGCTACCAGA	50.0	50.0	58.0	57.9	1252-1267 bp	0.6	65.7