Hotspot

ndhD

SSC · 113894-115396 · 1503 bp

Back to Barleria

Pi 0.0175

MarkerSeek score 57.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 51
pi: 0.0175
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2258-2261 bp	1.0	82.9
ndhD_p2	TTGGAGTTGGGACCCAAAAG	AAGATGTGAATCCGCCTGTC	50.0	50.0	57.9	58.0	2186-2189 bp	1.0	82.8
ndhD_p3	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.1	58.0	2280-2283 bp	1.0	82.6
ndhD_p4	CGCCAGGTCAAATTGCATTT	GATACCCTGGGACGGATGTA	45.0	55.0	57.9	58.0	1931-1933 bp	1.0	82.5
ndhD_p5	CGCCAGGTCAAATTGCATTT	AAGATGTGAATCCGCCTGTC	45.0	50.0	57.9	58.0	1859-1861 bp	1.0	82.4