Hotspot

ndhD-psaC

SSC · 115397-115525 · 129 bp

Back to Barleria

Pi 0.0548

MarkerSeek score 77.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 129
variable_sites: 13
pi: 0.0548
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCCGTCAGTAAAATGGGTCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.3	58.0	572-573 bp	1.0	82.3
ndhD-psaC_p2	CGAATCTCGAGTAACTGGCC	AAGATGTGAATCCGCCTGTC	55.0	50.0	58.2	58.0	552-553 bp	0.6	65.1
ndhD-psaC_p3	CGAATCTCGAGTAACTGGCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.2	58.0	624-625 bp	0.6	65.0
ndhD-psaC_p4	CGAATCTCGAGTAACTGGCC	AACAAATCGCTTCTGCTCCA	55.0	45.0	58.2	58.1	598-599 bp	0.6	64.3
ndhD-psaC_p5	CGAATCTCGAGTAACTGGCC	ACAAATCGCTTCTGCTCCAA	55.0	45.0	58.2	58.1	597-598 bp	0.6	64.3