Hotspot

trnL-UAG-ccsA

SSC · 112587-112669 · 83 bp

Back to Barleria

Pi 0.0313
MarkerSeek score 65.80
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
83
variable_sites
6
pi
0.0313
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TGGGTTAATATATGCTCTAAGGTTGA 50.0 34.6 57.9 57.7 178-179 bp 1.0 43.1
trnL-UAG-ccsA_p2 GGTAGACACGCTGCTCTTAG TGGGTTAATATATGCTCTAAGGTTGA 55.0 34.6 57.8 57.7 180-181 bp 1.0 43.1
trnL-UAG-ccsA_p3 GTAGACACGCTGCTCTTAGG TGGGTTAATATATGCTCTAAGGTTGA 55.0 34.6 57.8 57.7 179-180 bp 1.0 43.1
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TGGGTTAATATATGCTCTAAGGTTGA 45.0 34.6 57.8 57.7 185-186 bp 1.0 43.0
trnL-UAG-ccsA_p5 AATTGGTAGACACGCTGCTC TGGGTTAATATATGCTCTAAGGTTGA 50.0 34.6 58.3 57.7 184-185 bp 1.0 43.0