Hotspot

rpl32-trnL-UAG

SSC · 111736-112506 · 771 bp

Back to Barleria

Pi 0.0361
MarkerSeek score 74.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
771
variable_sites
54
pi
0.0361
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCGTTAA TTTAGGAGATGACATGCCGC 50.0 50.0 58.0 58.0 928-953 bp 1.0 87.6
rpl32-trnL-UAG_p2 GGGATATTGGGCAGCGTTAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 873-898 bp 1.0 87.4
rpl32-trnL-UAG_p3 GGGATATTGGGCAGCGTTAA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 866-891 bp 1.0 87.0
rpl32-trnL-UAG_p4 GGGATATTGGGCAGCGTTAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 871-896 bp 1.0 86.8
rpl32-trnL-UAG_p5 GGGATATTGGGCAGCGTTAA TGATCCACATAAAAGGGCCG 50.0 50.0 58.0 57.9 1463-1484 bp 0.6 66.6