Hotspot

trnT-UGU-trnL-UAA

LSC · 45937-46596 · 660 bp

Back to Barleria

Pi 0.0177

MarkerSeek score 58.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 24
pi: 0.0177
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TCGAAGATCCGTTTCGTGAC	GCTTCCATTGAGTCTCTGCA	50.0	50.0	58.0	57.9	1094-1111 bp	1.0	82.6
trnT-UGU-trnL-UAA_p2	TCGAAGATCCGTTTCGTGAC	TGCCCAAATACGTGTGTCAA	50.0	45.0	58.0	58.0	1569-1585 bp	1.0	82.4
trnT-UGU-trnL-UAA_p3	TCGAAGATCCGTTTCGTGAC	AGGAATTTGGACCCGCTAAC	50.0	50.0	58.0	57.9	1533-1549 bp	1.0	81.7
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TGCCCAAATACGTGTGTCAA	55.0	45.0	57.9	58.0	1366-1382 bp	1.0	81.2
trnT-UGU-trnL-UAA_p5	TCGAAGATCCGTTTCGTGAC	GAGTCTCTGCACCTATCCCT	50.0	55.0	58.0	57.9	1085-1095 bp	0.6	65.0