Hotspot

rpl16

LSC · 127916-129414 · 1499 bp

Back to Barfussia

Pi 0.00401

MarkerSeek score 61.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1499
variable_sites: 6
pi: 0.00401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCCAATACCCCTTTTTGT	TCGATTGATCATTTGGTATAATTGCT	45.0	30.8	56.6	57.6	1557-1559 bp	1.0	40.4
rpl16_p2	TCCCCAATACCCCTTTTTGTT	TCGATTGATCATTTGGTATAATTGCT	42.9	30.8	57.4	57.6	1557-1559 bp	1.0	40.4
rpl16_p3	TCCCCAATACCCCTTTTTGT	TCGATTGATCATTTGGTATAATTGC	45.0	32.0	56.6	56.4	1557-1559 bp	1.0	40.4
rpl16_p4	TCCCCAATACCCCTTTTTGT	CGATTGATCATTTGGTATAATTGCT	45.0	32.0	56.6	56.4	1556-1558 bp	1.0	40.4
rpl16_p5	ATCCCCAATACCCCTTTTTGT	TCGATTGATCATTTGGTATAATTGCT	42.9	30.8	57.1	57.6	1558-1560 bp	1.0	40.4