Hotspot

psbE-petL

LSC · 110866-112394 · 1529 bp

Back to Barfussia

Pi 0.00264

MarkerSeek score 54.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1529
variable_sites: 3
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1288-1676 bp	1.0	78.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1287-1675 bp	1.0	78.5
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1301-1689 bp	1.0	78.2
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1300-1688 bp	1.0	78.2
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.2	57.3	1296-1684 bp	1.0	77.5