Hotspot

trnF-GAA-ndhJ

LSC · 94284-95054 · 771 bp

Back to Barfussia

Pi 0.00131

MarkerSeek score 60.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 1
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	876-881 bp	1.0	79.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	866-871 bp	1.0	78.5
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	919-924 bp	1.0	77.8
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	918-923 bp	1.0	77.8
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.2	58.2	908-913 bp	1.0	77.3