Hotspot

rpoB-trnC-GCA

LSC · 72006-73355 · 1350 bp

Back to Barfussia

Pi 0.0037

MarkerSeek score 61.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1350
variable_sites: 4
pi: 0.0037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.4	58.1	1166-1417 bp	1.0	80.4
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.6	58.1	1167-1418 bp	1.0	80.2
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1192-1443 bp	1.0	77.2
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1193-1444 bp	1.0	76.9
rpoB-trnC-GCA_p5	TCTTCCGTCAAGCCTTGATT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.1	58.1	1243-1494 bp	1.0	76.8