Hotspot

trnS-GCU-trnG-UCC

LSC · 52656-53864 · 1209 bp

Back to Barfussia

Pi 0.0101

MarkerSeek score 54.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1209
variable_sites: 12
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	1336-1338 bp	1.0	53.4
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1281-1283 bp	1.0	53.3
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.0	57.3	1339-1341 bp	1.0	52.3
trnS-GCU-trnG-UCC_p4	GAGAAAGCGGAAAGAGAGGG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.0	57.3	1338-1340 bp	1.0	52.3
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1283-1285 bp	1.0	52.2