Hotspot

rpoA

Genome · 83888-84898 · 1011 bp

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Pi 0.00396

MarkerSeek score 48.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1011
variable_sites: 4
pi: 0.00396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GCCCAGTAGCTACAACAGTC	ATTCAAGGGCACAAGAAGGG	55.0	50.0	58.0	58.1	1896-1900 bp	1.0	79.9
rpoA_p2	GCCCAGTAGCTACAACAGTC	GTGGATTCAAGGGCACAAGA	55.0	50.0	58.0	58.1	1900-1904 bp	1.0	79.8
rpoA_p3	GCCCAGTAGCTACAACAGTC	AGAGATGCAGCATTACGAGC	55.0	50.0	58.0	58.1	1772-1776 bp	1.0	79.4
rpoA_p4	GCCCAGTAGCTACAACAGTC	CCATTTGCTGCTCAAACCAC	55.0	50.0	58.0	58.2	1871-1875 bp	1.0	78.8
rpoA_p5	GCCCAGTAGCTACAACAGTC	GTACCTGTGGATTCAAGGGC	55.0	55.0	58.0	58.2	1906-1910 bp	1.0	78.5