Hotspot

atpF

Genome · 13423-14714 · 1292 bp

Back to Barclaya

Pi 0.00311

MarkerSeek score 60.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 1292
variable_sites: 4
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCTTCGGCTCGAATGGTT	AAGTGTCTTATCGGGGATCT	45.0	45.0	57.8	55.3	1485-1490 bp	1.0	57.7
atpF_p2	TCTTCGGCTCGAATGGTTAC	AAGTGTCTTATCGGGGATCT	50.0	45.0	57.7	55.3	1483-1488 bp	1.0	56.8
atpF_p3	ATTTCTTCGGCTCGAATGGT	AAGTGTCTTATCGGGGATCT	45.0	45.0	57.6	55.3	1486-1491 bp	1.0	55.9
atpF_p4	TTTCTTCGGCTCGAATGGTT	TCGGGGATCTAAATAAGGAGTGA	45.0	43.5	57.8	58.2	1475-1480 bp	1.0	53.5
atpF_p5	CTTCGGCTCGAATGGTTACC	AAGTGTCTTATCGGGGATCT	55.0	45.0	58.7	55.3	1482-1487 bp	1.0	53.4