Hotspot

petD-rpoA

Genome · 83458-83887 · 430 bp

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Pi 0.0233

MarkerSeek score 68.60

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: Genome
length_bp: 430
variable_sites: 10
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCAGTAGCCCTTTGGTTAGG	GGAATTGAGCATTTTCGCGT	55.0	45.0	58.2	57.7	625-629 bp	1.0	77.2
petD-rpoA_p2	GCAGTAGCCCTTTGGTTAGG	GGGAATTGAGCATTTTCGCG	55.0	50.0	58.2	58.5	626-630 bp	1.0	75.7
petD-rpoA_p3	GCAGTAGCCCTTTGGTTAGG	TGGGAATTGAGCATTTTCGC	55.0	45.0	58.2	57.3	627-631 bp	1.0	74.1
petD-rpoA_p4	GCAGTAGCCCTTTGGTTAGG	TGAGCATTTTCGCGTAGAAGA	55.0	42.9	58.2	58.0	620-624 bp	1.0	70.9
petD-rpoA_p5	GCAGTAGCCCTTTGGTTAGG	ATGGGAATTGAGCATTTTCGC	55.0	42.9	58.2	57.8	628-632 bp	1.0	69.9