Hotspot

DR443_pgt018-atpE

Genome · 57170-57402 · 233 bp

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Pi 0.0182

MarkerSeek score 66.60

Primer available yes

Label DR443_pgt018-atpE

Feature Metadata

label_name: DR443_pgt018-atpE
feature_type: igs
region: Genome
length_bp: 233
variable_sites: 4
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR443_pgt018-atpE_p1	CGATACGATGCGCTCCATTA	GGCTATCACCGCTATTTCGT	50.0	50.0	58.0	57.8	363-373 bp	1.0	81.7
DR443_pgt018-atpE_p2	CGATACGATGCGCTCCATTA	AAGCTAACCTGAGGAAAGCG	50.0	50.0	58.0	57.9	445-455 bp	1.0	81.5
DR443_pgt018-atpE_p3	CGATACGATGCGCTCCATTA	TGGAGGCTATCACCGCTATT	50.0	50.0	58.0	58.3	367-377 bp	1.0	80.6
DR443_pgt018-atpE_p4	CGATACGATGCGCTCCATTA	AGGCTATCACCGCTATTTCG	50.0	50.0	58.0	57.6	364-374 bp	1.0	79.3
DR443_pgt018-atpE_p5	TATTGCTTTCATACGGCGGG	AAGCTAACCTGAGGAAAGCG	50.0	50.0	58.4	57.9	379-389 bp	1.0	78.8