Hotspot

DR443_pgt015-DR443_pgt016

Genome · 50404-51886 · 1483 bp

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Pi 0.0202

MarkerSeek score 72.60

Primer available yes

Label DR443_pgt015-DR443_pgt016

Feature Metadata

label_name: DR443_pgt015-DR443_pgt016
feature_type: igs
region: Genome
length_bp: 1483
variable_sites: 29
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR443_pgt015-DR443_pgt016_p1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	55.0	55.0	57.9	58.0	1514-1536 bp	1.0	82.4
DR443_pgt015-DR443_pgt016_p2	GAGTCGAACCGATGACCATC	TCTACCAGCTGAGCTATCCC	55.0	55.0	58.2	58.0	1545-1567 bp	1.0	82.2
DR443_pgt015-DR443_pgt016_p3	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1550-1572 bp	1.0	82.0
DR443_pgt015-DR443_pgt016_p4	GAGTCGAACCGATGACCATC	TTTGAACTGGTGACACGAGG	55.0	50.0	58.2	58.1	1581-1603 bp	1.0	81.7
DR443_pgt015-DR443_pgt016_p5	GCGATGCTCTAACCTCTGAG	TCAGTCCTCTGCTCTACCAG	55.0	55.0	57.9	57.9	1526-1548 bp	1.0	81.3