Hotspot

DR443_pgt011-psbZ

Genome · 39199-39572 · 374 bp

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Pi 0.00267
MarkerSeek score 48.60
Primer available yes
Label DR443_pgt011-psbZ

Feature Metadata

label_name
DR443_pgt011-psbZ
feature_type
igs
region
Genome
length_bp
374
variable_sites
1
pi
0.00267
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DR443_pgt011-psbZ_p1 CAACCACTCAGCCATCTCTC TACTTGACCAACCATCGGGA 55.0 50.0 58.0 58.4 501 bp 1.0 77.2
DR443_pgt011-psbZ_p2 GAGCTATCAACCACTCAGCC TACTTGACCAACCATCGGGA 55.0 50.0 58.1 58.4 508 bp 1.0 76.9
DR443_pgt011-psbZ_p3 GGAGCTATCAACCACTCAGC TACTTGACCAACCATCGGGA 55.0 50.0 58.1 58.4 509 bp 1.0 76.9
DR443_pgt011-psbZ_p4 CAAGACCGGAGCTATCAACC TACTTGACCAACCATCGGGA 55.0 50.0 58.1 58.4 516 bp 1.0 76.9
DR443_pgt011-psbZ_p5 TATTCCACGGGCAAAAGGAG TACTTGACCAACCATCGGGA 50.0 50.0 57.9 58.4 588 bp 1.0 76.3