Hotspot

DR443_pgt010-psbD

Genome · 34913-36464 · 1552 bp

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Pi 0.00644
MarkerSeek score 62.30
Primer available yes
Label DR443_pgt010-psbD

Feature Metadata

label_name
DR443_pgt010-psbD
feature_type
igs
region
Genome
length_bp
1552
variable_sites
10
pi
0.00644
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DR443_pgt010-psbD_p1 AACGGGCCCTTTTAACTCAG GAAGAGCAATAGACCGGACC 50.0 55.0 58.1 57.8 1746 bp 1.0 77.9
DR443_pgt010-psbD_p2 AACGGGCCCTTTTAACTCAG GGAAGAGCAATAGACCGGAC 50.0 55.0 58.1 57.8 1747 bp 1.0 77.9
DR443_pgt010-psbD_p3 GCCCTTTTAACTCAGCGGTA GAAGAGCAATAGACCGGACC 50.0 55.0 57.9 57.8 1741 bp 1.0 77.8
DR443_pgt010-psbD_p4 GCCCTTTTAACTCAGCGGTA GGAAGAGCAATAGACCGGAC 50.0 55.0 57.9 57.8 1742 bp 1.0 77.8
DR443_pgt010-psbD_p5 AACGGGCCCTTTTAACTCAG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1721 bp 1.0 77.7