Hotspot

DR443_pgt008-DR443_pgt009

Genome · 33532-33947 · 416 bp

Back to Barclaya

Pi 0.012

MarkerSeek score 57.60

Primer available yes

Label DR443_pgt008-DR443_pgt009

Feature Metadata

label_name: DR443_pgt008-DR443_pgt009
feature_type: igs
region: Genome
length_bp: 416
variable_sites: 5
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR443_pgt008-DR443_pgt009_p1	TTCATAGGATCCCTGGTCGG	GGGGACGGACTGTAAATTCG	55.0	55.0	58.3	58.4	573-578 bp	1.0	75.8
DR443_pgt008-DR443_pgt009_p2	CATAGGATCCCTGGTCGGTA	GGGGACGGACTGTAAATTCG	55.0	55.0	57.7	58.4	571-576 bp	1.0	75.6
DR443_pgt008-DR443_pgt009_p3	ATAGGATCCCTGGTCGGTAC	GGGGACGGACTGTAAATTCG	55.0	55.0	57.7	58.4	570-575 bp	1.0	75.6
DR443_pgt008-DR443_pgt009_p4	TTCATAGGATCCCTGGTCGG	GGGACGGACTGTAAATTCGT	55.0	50.0	58.3	57.6	572-577 bp	1.0	75.6
DR443_pgt008-DR443_pgt009_p5	CATAGGATCCCTGGTCGGTA	GGGACGGACTGTAAATTCGT	55.0	50.0	57.7	57.6	570-575 bp	1.0	75.4