Hotspot

petN-psbM

Genome · 31237-32558 · 1322 bp

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Pi 0.00758

MarkerSeek score 63.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1322
variable_sites: 10
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GAAAGGGGGCATGATTCACA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.1	58.3	1512 bp	1.0	77.3
petN-psbM_p2	AGAAAGGGGGCATGATTCAC	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.9	58.3	1513 bp	1.0	77.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1486 bp	1.0	77.3
petN-psbM_p4	GAAAGGGGGCATGATTCACA	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.1	57.6	1513 bp	1.0	76.3
petN-psbM_p5	AGAAAGGGGGCATGATTCAC	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.9	57.6	1514 bp	1.0	76.3