Hotspot

matK-DR443_pgt002

Genome · 3737-4479 · 743 bp

Back to Barclaya

Pi 0.00538

MarkerSeek score 61.80

Primer available yes

Label matK-DR443_pgt002

Feature Metadata

label_name: matK-DR443_pgt002
feature_type: igs
region: Genome
length_bp: 743
variable_sites: 4
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-DR443_pgt002_p1	CGCTGTTTCCGGTATCTCTC	TTTGACTTGACTGGTTCCGG	55.0	50.0	58.2	58.0	910-916 bp	1.0	78.8
matK-DR443_pgt002_p2	TATAGGAAGCGCTGTTTCCG	TTTGACTTGACTGGTTCCGG	50.0	50.0	57.8	58.0	919-925 bp	1.0	78.3
matK-DR443_pgt002_p3	CGCTGTTTCCGGTATCTCTC	TCTGGGTTGCTAACTCAACG	55.0	50.0	58.2	57.8	845-851 bp	1.0	77.8
matK-DR443_pgt002_p4	CGCTGTTTCCGGTATCTCTC	AACGGTAGAGTACTCGGCTT	55.0	50.0	58.2	58.2	829-835 bp	1.0	77.8
matK-DR443_pgt002_p5	CGCTGTTTCCGGTATCTCTC	ACGGTAGAGTACTCGGCTTT	55.0	50.0	58.2	58.2	828-834 bp	1.0	77.8