Hotspot

rpoC2

LSC · 15909-20048 · 4140 bp

Back to Barbarea

Pi 0.00451

MarkerSeek score 43.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4140
variable_sites: 28
pi: 0.00451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTTCCAACCCTTAATCCGCT	AAGGCTGGCATTCTTCAACA	50.0	45.0	57.9	58.0	792 bp	0.666667	66.4
rpoC2_p2	GTTCCAACCCTTAATCCGCT	AGGCTGGCATTCTTCAACAA	50.0	45.0	57.9	58.0	791 bp	0.666667	66.4
rpoC2_p3	TATTGAATCAATCGCGCGGA	AAGGCTGGCATTCTTCAACA	45.0	45.0	58.1	58.0	756 bp	0.666667	66.2
rpoC2_p4	TATTGAATCAATCGCGCGGA	AGGCTGGCATTCTTCAACAA	45.0	45.0	58.1	58.0	755 bp	0.666667	66.2
rpoC2_p5	GCACCAATCAAGAATCCCCA	AGGCTGGCATTCTTCAACAA	50.0	45.0	58.1	58.0	841 bp	0.666667	66.1