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ndhF-rpl32

SSC · 112517-113376 · 860 bp

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Pi 0.00938

MarkerSeek score 68.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 860
variable_sites: 12
pi: 0.00938
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGGAGTGGAATGAAAGGG	AGCTTTCAACGACGTCCAAT	55.0	45.0	57.9	58.1	975-1027 bp	1.0	81.9
ndhF-rpl32_p2	CTGGGAGTGGAATGAAAGGG	CGACGTCCAATATCCCTTCC	55.0	55.0	57.9	57.8	966-1018 bp	1.0	81.7
ndhF-rpl32_p3	TGGGAGTGGAATGAAAGGGA	AGCTTTCAACGACGTCCAAT	50.0	45.0	58.2	58.1	974-1026 bp	1.0	81.0
ndhF-rpl32_p4	TGGGAGTGGAATGAAAGGGA	CGACGTCCAATATCCCTTCC	50.0	55.0	58.2	57.8	965-1017 bp	1.0	80.8
ndhF-rpl32_p5	CTGGGAGTGGAATGAAAGGG	AAGCTTTCAACGACGTCCAA	55.0	45.0	57.9	58.3	976-1028 bp	1.0	80.1