Hotspot

rbcL-accD

LSC · 56335-56987 · 653 bp

Back to Barbarea

Pi 0.00919

MarkerSeek score 64.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 9
pi: 0.00919
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	TAGCTCGCCTCTGTATTCCA	55.0	50.0	57.7	57.9	804-805 bp	1.0	78.0
rbcL-accD_p2	GCTTGCAAATGGAGTCCTGA	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.5	57.9	815-816 bp	1.0	76.6
rbcL-accD_p3	TGAGGCTTGCAAATGGAGTC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.5	57.9	819-820 bp	1.0	76.6
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	GCTCGCCTCTGTATTCCAAT	55.0	50.0	57.7	57.8	802-803 bp	1.0	76.6
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	TGCTTTACTTAGCTCGCCTC	55.0	50.0	57.7	57.7	813-814 bp	1.0	76.2