Hotspot

trnF-GAA-ndhJ

LSC · 47944-48526 · 583 bp

Back to Barbarea

Pi 0.012

MarkerSeek score 66.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 10
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	722-723 bp	1.0	80.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	695-696 bp	1.0	80.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	694-695 bp	1.0	80.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	702-703 bp	1.0	79.9
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	703-704 bp	1.0	79.9