Hotspot

rpoB-trnC-GCA

LSC · 26311-27411 · 1101 bp

Back to Barbarea

Pi 0.00848

MarkerSeek score 53.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 14
pi: 0.00848
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCCCTCTTTTCCATCCCC	CTTACCACTCGGCCATGC	55.0	61.1	57.9	58.2	1145-1149 bp	1.0	63.0
rpoB-trnC-GCA_p2	GTTCCCTCTTTTCCATCCCC	CCTTACCACTCGGCCATG	55.0	61.1	57.9	57.5	1146-1150 bp	1.0	60.2
rpoB-trnC-GCA_p3	TGTTCCCTCTTTTCCATCCC	CTTACCACTCGGCCATGC	50.0	61.1	57.1	58.2	1146-1150 bp	1.0	57.0
rpoB-trnC-GCA_p4	TCTTTTCCATCCCCGAGCAT	CTTACCACTCGGCCATGC	50.0	61.1	59.1	58.2	1139-1143 bp	1.0	55.5
rpoB-trnC-GCA_p5	CTCTTTTCCATCCCCGAGC	CTTACCACTCGGCCATGC	57.9	61.1	57.9	58.2	1140-1144 bp	1.0	55.5