Hotspot

trnR-UCU-atpA

LSC · 9601-9899 · 299 bp

Back to Barbarea

Pi 0.0119

MarkerSeek score 61.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 5
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	52.6	45.0	58.0	57.7	405-421 bp	1.0	73.8
trnR-UCU-atpA_p2	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	52.6	45.0	58.0	57.7	406-422 bp	1.0	73.8
trnR-UCU-atpA_p3	GCGAAAAGCGTCCATTGTC	ACCGCAGTTCCAAGAAATCA	52.6	45.0	58.0	57.7	480-496 bp	1.0	73.6
trnR-UCU-atpA_p4	GCGAAAAGCGTCCATTGTC	ACATTAACCGCTGAAGCAGA	52.6	45.0	58.0	57.5	446-462 bp	1.0	72.3
trnR-UCU-atpA_p5	GCGAAAAGCGTCCATTGTC	AGACATTAACCGCTGAAGCA	52.6	45.0	58.0	57.5	448-464 bp	1.0	72.2