Hotspot

trnS-GCU-trnG

LSC · 7926-8581 · 656 bp

Back to Barbarea

Pi 0.0251

MarkerSeek score 67.20

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 24
pi: 0.0251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCA	50.0	40.9	57.8	56.9	718-726 bp	1.0	57.8
trnS-GCU-trnG_p2	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCA	50.0	40.9	58.2	56.9	716-724 bp	1.0	57.5
trnS-GCU-trnG_p3	CCAACGCTTTAGTCCACTCA	AGGGATTAAAGGAACGAATCACA	50.0	39.1	57.8	57.8	728-736 bp	1.0	57.0
trnS-GCU-trnG_p4	AACGCTTTAGTCCACTCAGC	AGGGATTAAAGGAACGAATCACA	50.0	39.1	58.2	57.8	726-734 bp	1.0	56.7
trnS-GCU-trnG_p5	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCAC	50.0	43.5	57.8	58.2	718-726 bp	1.0	56.7